NO!
TrinketAfterbirthUnlocked
How do you unlock NO!?
Verified starting unlockSource data confirms that no unlock is required.
Prevents active items from being generated, if possible.
Available from the start
No unlock needed — this is available in every new save file.
What unlocking NO! leads to
Nothing depends on this yet.
Source: bindingofisaacrebirth.wiki.gg
Imported 2026-09-13