Death Certificate

AchievementRepentanceUnlock unknown

Achievement details

Achievement number: 636
Description: ...
Requirement: Earn all Hard mode Completion Marks for all characters, including tainted ones

How do you unlock Death Certificate?

In The Binding of Isaac, Death Certificate is unlocked by earning the Mom's Heart or It Lives! completion mark as Isaac (Character) on hard mode, earning the Isaac completion mark as Isaac (Character) on hard mode, and earning the ??? completion mark as Isaac (Character) on hard mode, and 405 more requirements.

Structured pathSource-backed requirements are mapped into trackable steps.

...

See the full unlock path for Death Certificate — 485 steps

All of
- Isaac (Character) → Mom's Heart or It Lives! (hard)
  - Isaac (Character)
- Isaac (Character) → Isaac (hard)
  - Isaac (Character)
- Isaac (Character) → ??? (hard)
  - Isaac (Character)
- Isaac (Character) → Satan (hard)
  - Isaac (Character)
- Isaac (Character) → The Lamb (hard)
  1. Defeat Satan 5 times to unlock The Negative. source
  2. The Negative is a guaranteed drop from Mom; take it, then defeat Satan in Sheol and touch the chest to enter Dark Room. source
  3. Find and defeat The Lamb in Dark Room. source
  - Isaac (Character)
- Isaac (Character) → Mega Satan (hard)
  1. ??? or The Lamb must be defeated once for the Golden Gate to spawn. source
  2. Open the Golden Gate by combining Key Piece 1 and Key Piece 2 into the Golden Key, or by using Dad's Key or another gate-opening effect. source
  3. Reach the starting room of Chest or Dark Room, pass through the Golden Gate, and defeat Mega Satan. source
  - Isaac (Character)
- Isaac (Character) → Boss Rush (hard)
  - Isaac (Character)
- Isaac (Character) → Hush (hard)
  1. Defeat Mom's Heart for the 10th time to unlock Blue Womb. source
  2. Defeat Mom's Heart or It Lives! within 30 minutes and enter the door to Blue Womb. source
  3. Defeat Hush in Blue Womb. source
  - Isaac (Character)
- Isaac (Character) → Ultra Greedier (hard)
  - Isaac (Character)
- Isaac (Character) → Delirium (hard)
  1. Defeat Hush to unlock The Void. source
  2. A Void portal can appear after a major boss; defeating Hush guarantees one. source
  3. Defeat Delirium, the main boss of The Void. source
  - Isaac (Character)
- Isaac (Character) → Mother (hard)
  1. Defeat Hush 3 times to unlock A Secret Exit. source
  2. After A Secret Exit is unlocked, a locked door to Downpour appears after completing Basement/Cellar/Burning Basement I or II. source
  3. Collect Knife Piece 1 in Downpour/Dross II. source
  4. Collect Knife Piece 2 in Mines/Ashpit II. source
  5. After defeating Mom in Mausoleum/Gehenna II, stab the flesh door that appears with the knife familiar to open it. source
  6. Defeat the Mausoleum version of Mom's Heart behind the flesh door, then reach Corpse II and defeat Mother. source
  - Isaac (Character)
- Isaac (Character) → The Beast (hard)
  1. A Strange Door is unlocked by beating Mother for the first time. source
  2. Take The Polaroid or The Negative after Mom, escape the boss room, then use it to open A Strange Door. source
  3. Find Dad's Note, complete the Ascent to Home, then defeat Dogma, the Ultra Harbingers, and The Beast. source
  - Isaac (Character)
- Magdalene (Character) → Mom's Heart or It Lives! (hard)
  - Magdalene (Character)
- Magdalene (Character) → Isaac (hard)
  - Magdalene (Character)
- Magdalene (Character) → ??? (hard)
  - Magdalene (Character)
- Magdalene (Character) → Satan (hard)
  - Magdalene (Character)
- Magdalene (Character) → The Lamb (hard)
  1. Defeat Satan 5 times to unlock The Negative. source
  2. The Negative is a guaranteed drop from Mom; take it, then defeat Satan in Sheol and touch the chest to enter Dark Room. source
  3. Find and defeat The Lamb in Dark Room. source
  - Magdalene (Character)
- Magdalene (Character) → Mega Satan (hard)
  1. ??? or The Lamb must be defeated once for the Golden Gate to spawn. source
  2. Open the Golden Gate by combining Key Piece 1 and Key Piece 2 into the Golden Key, or by using Dad's Key or another gate-opening effect. source
  3. Reach the starting room of Chest or Dark Room, pass through the Golden Gate, and defeat Mega Satan. source
  - Magdalene (Character)
- Magdalene (Character) → Boss Rush (hard)
  - Magdalene (Character)
- Magdalene (Character) → Hush (hard)
  1. Defeat Mom's Heart for the 10th time to unlock Blue Womb. source
  2. Defeat Mom's Heart or It Lives! within 30 minutes and enter the door to Blue Womb. source
  3. Defeat Hush in Blue Womb. source
  - Magdalene (Character)
- Magdalene (Character) → Ultra Greedier (hard)
  - Magdalene (Character)
- Magdalene (Character) → Delirium (hard)
  1. Defeat Hush to unlock The Void. source
  2. A Void portal can appear after a major boss; defeating Hush guarantees one. source
  3. Defeat Delirium, the main boss of The Void. source
  - Magdalene (Character)
- Magdalene (Character) → Mother (hard)
  1. Defeat Hush 3 times to unlock A Secret Exit. source
  2. After A Secret Exit is unlocked, a locked door to Downpour appears after completing Basement/Cellar/Burning Basement I or II. source
  3. Collect Knife Piece 1 in Downpour/Dross II. source
  4. Collect Knife Piece 2 in Mines/Ashpit II. source
  5. After defeating Mom in Mausoleum/Gehenna II, stab the flesh door that appears with the knife familiar to open it. source
  6. Defeat the Mausoleum version of Mom's Heart behind the flesh door, then reach Corpse II and defeat Mother. source
  - Magdalene (Character)
- Magdalene (Character) → The Beast (hard)
  1. A Strange Door is unlocked by beating Mother for the first time. source
  2. Take The Polaroid or The Negative after Mom, escape the boss room, then use it to open A Strange Door. source
  3. Find Dad's Note, complete the Ascent to Home, then defeat Dogma, the Ultra Harbingers, and The Beast. source
  - Magdalene (Character)
- Cain (Character) → Mom's Heart or It Lives! (hard)
  - Cain (Character)
- Cain (Character) → Isaac (hard)
  - Cain (Character)
- Cain (Character) → ??? (hard)
  - Cain (Character)
- Cain (Character) → Satan (hard)
  - Cain (Character)
- Cain (Character) → The Lamb (hard)
  1. Defeat Satan 5 times to unlock The Negative. source
  2. The Negative is a guaranteed drop from Mom; take it, then defeat Satan in Sheol and touch the chest to enter Dark Room. source
  3. Find and defeat The Lamb in Dark Room. source
  - Cain (Character)
- Cain (Character) → Mega Satan (hard)
  1. ??? or The Lamb must be defeated once for the Golden Gate to spawn. source
  2. Open the Golden Gate by combining Key Piece 1 and Key Piece 2 into the Golden Key, or by using Dad's Key or another gate-opening effect. source
  3. Reach the starting room of Chest or Dark Room, pass through the Golden Gate, and defeat Mega Satan. source
  - Cain (Character)
- Cain (Character) → Boss Rush (hard)
  - Cain (Character)
- Cain (Character) → Hush (hard)
  1. Defeat Mom's Heart for the 10th time to unlock Blue Womb. source
  2. Defeat Mom's Heart or It Lives! within 30 minutes and enter the door to Blue Womb. source
  3. Defeat Hush in Blue Womb. source
  - Cain (Character)
- Cain (Character) → Ultra Greedier (hard)
  - Cain (Character)
- Cain (Character) → Delirium (hard)
  1. Defeat Hush to unlock The Void. source
  2. A Void portal can appear after a major boss; defeating Hush guarantees one. source
  3. Defeat Delirium, the main boss of The Void. source
  - Cain (Character)
- Cain (Character) → Mother (hard)
  1. Defeat Hush 3 times to unlock A Secret Exit. source
  2. After A Secret Exit is unlocked, a locked door to Downpour appears after completing Basement/Cellar/Burning Basement I or II. source
  3. Collect Knife Piece 1 in Downpour/Dross II. source
  4. Collect Knife Piece 2 in Mines/Ashpit II. source
  5. After defeating Mom in Mausoleum/Gehenna II, stab the flesh door that appears with the knife familiar to open it. source
  6. Defeat the Mausoleum version of Mom's Heart behind the flesh door, then reach Corpse II and defeat Mother. source
  - Cain (Character)
- Cain (Character) → The Beast (hard)
  1. A Strange Door is unlocked by beating Mother for the first time. source
  2. Take The Polaroid or The Negative after Mom, escape the boss room, then use it to open A Strange Door. source
  3. Find Dad's Note, complete the Ascent to Home, then defeat Dogma, the Ultra Harbingers, and The Beast. source
  - Cain (Character)
- Judas (Character) → Mom's Heart or It Lives! (hard)
  - Judas (Character)
- Judas (Character) → Isaac (hard)
  - Judas (Character)
- Judas (Character) → ??? (hard)
  - Judas (Character)
- Judas (Character) → Satan (hard)
  - Judas (Character)
- Judas (Character) → The Lamb (hard)
  1. Defeat Satan 5 times to unlock The Negative. source
  2. The Negative is a guaranteed drop from Mom; take it, then defeat Satan in Sheol and touch the chest to enter Dark Room. source
  3. Find and defeat The Lamb in Dark Room. source
  - Judas (Character)
- Judas (Character) → Mega Satan (hard)
  1. ??? or The Lamb must be defeated once for the Golden Gate to spawn. source
  2. Open the Golden Gate by combining Key Piece 1 and Key Piece 2 into the Golden Key, or by using Dad's Key or another gate-opening effect. source
  3. Reach the starting room of Chest or Dark Room, pass through the Golden Gate, and defeat Mega Satan. source
  - Judas (Character)
- Judas (Character) → Boss Rush (hard)
  - Judas (Character)
- Judas (Character) → Hush (hard)
  1. Defeat Mom's Heart for the 10th time to unlock Blue Womb. source
  2. Defeat Mom's Heart or It Lives! within 30 minutes and enter the door to Blue Womb. source
  3. Defeat Hush in Blue Womb. source
  - Judas (Character)
- Judas (Character) → Ultra Greedier (hard)
  - Judas (Character)
- Judas (Character) → Delirium (hard)
  1. Defeat Hush to unlock The Void. source
  2. A Void portal can appear after a major boss; defeating Hush guarantees one. source
  3. Defeat Delirium, the main boss of The Void. source
  - Judas (Character)
- Judas (Character) → Mother (hard)
  1. Defeat Hush 3 times to unlock A Secret Exit. source
  2. After A Secret Exit is unlocked, a locked door to Downpour appears after completing Basement/Cellar/Burning Basement I or II. source
  3. Collect Knife Piece 1 in Downpour/Dross II. source
  4. Collect Knife Piece 2 in Mines/Ashpit II. source
  5. After defeating Mom in Mausoleum/Gehenna II, stab the flesh door that appears with the knife familiar to open it. source
  6. Defeat the Mausoleum version of Mom's Heart behind the flesh door, then reach Corpse II and defeat Mother. source
  - Judas (Character)
- Judas (Character) → The Beast (hard)
  1. A Strange Door is unlocked by beating Mother for the first time. source
  2. Take The Polaroid or The Negative after Mom, escape the boss room, then use it to open A Strange Door. source
  3. Find Dad's Note, complete the Ascent to Home, then defeat Dogma, the Ultra Harbingers, and The Beast. source
  - Judas (Character)
- ??? (Character) → Mom's Heart or It Lives! (hard)
  - ??? (Character)
- ??? (Character) → Isaac (hard)
  - ??? (Character)
- ??? (Character) → ??? (hard)
  - ??? (Character)
- ??? (Character) → Satan (hard)
  - ??? (Character)
- ??? (Character) → The Lamb (hard)
  1. Defeat Satan 5 times to unlock The Negative. source
  2. The Negative is a guaranteed drop from Mom; take it, then defeat Satan in Sheol and touch the chest to enter Dark Room. source
  3. Find and defeat The Lamb in Dark Room. source
  - ??? (Character)
- ??? (Character) → Mega Satan (hard)
  1. ??? or The Lamb must be defeated once for the Golden Gate to spawn. source
  2. Open the Golden Gate by combining Key Piece 1 and Key Piece 2 into the Golden Key, or by using Dad's Key or another gate-opening effect. source
  3. Reach the starting room of Chest or Dark Room, pass through the Golden Gate, and defeat Mega Satan. source
  - ??? (Character)
- ??? (Character) → Boss Rush (hard)
  - ??? (Character)
- ??? (Character) → Hush (hard)
  1. Defeat Mom's Heart for the 10th time to unlock Blue Womb. source
  2. Defeat Mom's Heart or It Lives! within 30 minutes and enter the door to Blue Womb. source
  3. Defeat Hush in Blue Womb. source
  - ??? (Character)
- ??? (Character) → Ultra Greedier (hard)
  - ??? (Character)
- ??? (Character) → Delirium (hard)
  1. Defeat Hush to unlock The Void. source
  2. A Void portal can appear after a major boss; defeating Hush guarantees one. source
  3. Defeat Delirium, the main boss of The Void. source
  - ??? (Character)
- ??? (Character) → Mother (hard)
  1. Defeat Hush 3 times to unlock A Secret Exit. source
  2. After A Secret Exit is unlocked, a locked door to Downpour appears after completing Basement/Cellar/Burning Basement I or II. source
  3. Collect Knife Piece 1 in Downpour/Dross II. source
  4. Collect Knife Piece 2 in Mines/Ashpit II. source
  5. After defeating Mom in Mausoleum/Gehenna II, stab the flesh door that appears with the knife familiar to open it. source
  6. Defeat the Mausoleum version of Mom's Heart behind the flesh door, then reach Corpse II and defeat Mother. source
  - ??? (Character)
- ??? (Character) → The Beast (hard)
  1. A Strange Door is unlocked by beating Mother for the first time. source
  2. Take The Polaroid or The Negative after Mom, escape the boss room, then use it to open A Strange Door. source
  3. Find Dad's Note, complete the Ascent to Home, then defeat Dogma, the Ultra Harbingers, and The Beast. source
  - ??? (Character)
- Eve (Character) → Mom's Heart or It Lives! (hard)
  - Eve (Character)
- Eve (Character) → Isaac (hard)
  - Eve (Character)
- Eve (Character) → ??? (hard)
  - Eve (Character)
- Eve (Character) → Satan (hard)
  - Eve (Character)
- Eve (Character) → The Lamb (hard)
  1. Defeat Satan 5 times to unlock The Negative. source
  2. The Negative is a guaranteed drop from Mom; take it, then defeat Satan in Sheol and touch the chest to enter Dark Room. source
  3. Find and defeat The Lamb in Dark Room. source
  - Eve (Character)
- Eve (Character) → Mega Satan (hard)
  1. ??? or The Lamb must be defeated once for the Golden Gate to spawn. source
  2. Open the Golden Gate by combining Key Piece 1 and Key Piece 2 into the Golden Key, or by using Dad's Key or another gate-opening effect. source
  3. Reach the starting room of Chest or Dark Room, pass through the Golden Gate, and defeat Mega Satan. source
  - Eve (Character)
- Eve (Character) → Boss Rush (hard)
  - Eve (Character)
- Eve (Character) → Hush (hard)
  1. Defeat Mom's Heart for the 10th time to unlock Blue Womb. source
  2. Defeat Mom's Heart or It Lives! within 30 minutes and enter the door to Blue Womb. source
  3. Defeat Hush in Blue Womb. source
  - Eve (Character)
- Eve (Character) → Ultra Greedier (hard)
  - Eve (Character)
- Eve (Character) → Delirium (hard)
  1. Defeat Hush to unlock The Void. source
  2. A Void portal can appear after a major boss; defeating Hush guarantees one. source
  3. Defeat Delirium, the main boss of The Void. source
  - Eve (Character)
- Eve (Character) → Mother (hard)
  1. Defeat Hush 3 times to unlock A Secret Exit. source
  2. After A Secret Exit is unlocked, a locked door to Downpour appears after completing Basement/Cellar/Burning Basement I or II. source
  3. Collect Knife Piece 1 in Downpour/Dross II. source
  4. Collect Knife Piece 2 in Mines/Ashpit II. source
  5. After defeating Mom in Mausoleum/Gehenna II, stab the flesh door that appears with the knife familiar to open it. source
  6. Defeat the Mausoleum version of Mom's Heart behind the flesh door, then reach Corpse II and defeat Mother. source
  - Eve (Character)
- Eve (Character) → The Beast (hard)
  1. A Strange Door is unlocked by beating Mother for the first time. source
  2. Take The Polaroid or The Negative after Mom, escape the boss room, then use it to open A Strange Door. source
  3. Find Dad's Note, complete the Ascent to Home, then defeat Dogma, the Ultra Harbingers, and The Beast. source
  - Eve (Character)
- Samson (Character) → Mom's Heart or It Lives! (hard)
  - Samson (Character)
- Samson (Character) → Isaac (hard)
  - Samson (Character)
- Samson (Character) → ??? (hard)
  - Samson (Character)
- Samson (Character) → Satan (hard)
  - Samson (Character)
- Samson (Character) → The Lamb (hard)
  1. Defeat Satan 5 times to unlock The Negative. source
  2. The Negative is a guaranteed drop from Mom; take it, then defeat Satan in Sheol and touch the chest to enter Dark Room. source
  3. Find and defeat The Lamb in Dark Room. source
  - Samson (Character)
- Samson (Character) → Mega Satan (hard)
  1. ??? or The Lamb must be defeated once for the Golden Gate to spawn. source
  2. Open the Golden Gate by combining Key Piece 1 and Key Piece 2 into the Golden Key, or by using Dad's Key or another gate-opening effect. source
  3. Reach the starting room of Chest or Dark Room, pass through the Golden Gate, and defeat Mega Satan. source
  - Samson (Character)
- Samson (Character) → Boss Rush (hard)
  - Samson (Character)
- Samson (Character) → Hush (hard)
  1. Defeat Mom's Heart for the 10th time to unlock Blue Womb. source
  2. Defeat Mom's Heart or It Lives! within 30 minutes and enter the door to Blue Womb. source
  3. Defeat Hush in Blue Womb. source
  - Samson (Character)
- Samson (Character) → Ultra Greedier (hard)
  - Samson (Character)
- Samson (Character) → Delirium (hard)
  1. Defeat Hush to unlock The Void. source
  2. A Void portal can appear after a major boss; defeating Hush guarantees one. source
  3. Defeat Delirium, the main boss of The Void. source
  - Samson (Character)
- Samson (Character) → Mother (hard)
  1. Defeat Hush 3 times to unlock A Secret Exit. source
  2. After A Secret Exit is unlocked, a locked door to Downpour appears after completing Basement/Cellar/Burning Basement I or II. source
  3. Collect Knife Piece 1 in Downpour/Dross II. source
  4. Collect Knife Piece 2 in Mines/Ashpit II. source
  5. After defeating Mom in Mausoleum/Gehenna II, stab the flesh door that appears with the knife familiar to open it. source
  6. Defeat the Mausoleum version of Mom's Heart behind the flesh door, then reach Corpse II and defeat Mother. source
  - Samson (Character)
- Samson (Character) → The Beast (hard)
  1. A Strange Door is unlocked by beating Mother for the first time. source
  2. Take The Polaroid or The Negative after Mom, escape the boss room, then use it to open A Strange Door. source
  3. Find Dad's Note, complete the Ascent to Home, then defeat Dogma, the Ultra Harbingers, and The Beast. source
  - Samson (Character)
- Azazel (Character) → Mom's Heart or It Lives! (hard)
  - Azazel (Character)
- Azazel (Character) → Isaac (hard)
  - Azazel (Character)
- Azazel (Character) → ??? (hard)
  - Azazel (Character)
- Azazel (Character) → Satan (hard)
  - Azazel (Character)
- Azazel (Character) → The Lamb (hard)
  1. Defeat Satan 5 times to unlock The Negative. source
  2. The Negative is a guaranteed drop from Mom; take it, then defeat Satan in Sheol and touch the chest to enter Dark Room. source
  3. Find and defeat The Lamb in Dark Room. source
  - Azazel (Character)
- Azazel (Character) → Mega Satan (hard)
  1. ??? or The Lamb must be defeated once for the Golden Gate to spawn. source
  2. Open the Golden Gate by combining Key Piece 1 and Key Piece 2 into the Golden Key, or by using Dad's Key or another gate-opening effect. source
  3. Reach the starting room of Chest or Dark Room, pass through the Golden Gate, and defeat Mega Satan. source
  - Azazel (Character)
- Azazel (Character) → Boss Rush (hard)
  - Azazel (Character)
- Azazel (Character) → Hush (hard)
  1. Defeat Mom's Heart for the 10th time to unlock Blue Womb. source
  2. Defeat Mom's Heart or It Lives! within 30 minutes and enter the door to Blue Womb. source
  3. Defeat Hush in Blue Womb. source
  - Azazel (Character)
- Azazel (Character) → Ultra Greedier (hard)
  - Azazel (Character)
- Azazel (Character) → Delirium (hard)
  1. Defeat Hush to unlock The Void. source
  2. A Void portal can appear after a major boss; defeating Hush guarantees one. source
  3. Defeat Delirium, the main boss of The Void. source
  - Azazel (Character)
- Azazel (Character) → Mother (hard)
  1. Defeat Hush 3 times to unlock A Secret Exit. source
  2. After A Secret Exit is unlocked, a locked door to Downpour appears after completing Basement/Cellar/Burning Basement I or II. source
  3. Collect Knife Piece 1 in Downpour/Dross II. source
  4. Collect Knife Piece 2 in Mines/Ashpit II. source
  5. After defeating Mom in Mausoleum/Gehenna II, stab the flesh door that appears with the knife familiar to open it. source
  6. Defeat the Mausoleum version of Mom's Heart behind the flesh door, then reach Corpse II and defeat Mother. source
  - Azazel (Character)
- Azazel (Character) → The Beast (hard)
  1. A Strange Door is unlocked by beating Mother for the first time. source
  2. Take The Polaroid or The Negative after Mom, escape the boss room, then use it to open A Strange Door. source
  3. Find Dad's Note, complete the Ascent to Home, then defeat Dogma, the Ultra Harbingers, and The Beast. source
  - Azazel (Character)
- Lazarus (Character) → Mom's Heart or It Lives! (hard)
  - Lazarus (Character)
- Lazarus (Character) → Isaac (hard)
  - Lazarus (Character)
- Lazarus (Character) → ??? (hard)
  - Lazarus (Character)
- Lazarus (Character) → Satan (hard)
  - Lazarus (Character)
- Lazarus (Character) → The Lamb (hard)
  1. Defeat Satan 5 times to unlock The Negative. source
  2. The Negative is a guaranteed drop from Mom; take it, then defeat Satan in Sheol and touch the chest to enter Dark Room. source
  3. Find and defeat The Lamb in Dark Room. source
  - Lazarus (Character)
- Lazarus (Character) → Mega Satan (hard)
  1. ??? or The Lamb must be defeated once for the Golden Gate to spawn. source
  2. Open the Golden Gate by combining Key Piece 1 and Key Piece 2 into the Golden Key, or by using Dad's Key or another gate-opening effect. source
  3. Reach the starting room of Chest or Dark Room, pass through the Golden Gate, and defeat Mega Satan. source
  - Lazarus (Character)
- Lazarus (Character) → Boss Rush (hard)
  - Lazarus (Character)
- Lazarus (Character) → Hush (hard)
  1. Defeat Mom's Heart for the 10th time to unlock Blue Womb. source
  2. Defeat Mom's Heart or It Lives! within 30 minutes and enter the door to Blue Womb. source
  3. Defeat Hush in Blue Womb. source
  - Lazarus (Character)
- Lazarus (Character) → Ultra Greedier (hard)
  - Lazarus (Character)
- Lazarus (Character) → Delirium (hard)
  1. Defeat Hush to unlock The Void. source
  2. A Void portal can appear after a major boss; defeating Hush guarantees one. source
  3. Defeat Delirium, the main boss of The Void. source
  - Lazarus (Character)
- Lazarus (Character) → Mother (hard)
  1. Defeat Hush 3 times to unlock A Secret Exit. source
  2. After A Secret Exit is unlocked, a locked door to Downpour appears after completing Basement/Cellar/Burning Basement I or II. source
  3. Collect Knife Piece 1 in Downpour/Dross II. source
  4. Collect Knife Piece 2 in Mines/Ashpit II. source
  5. After defeating Mom in Mausoleum/Gehenna II, stab the flesh door that appears with the knife familiar to open it. source
  6. Defeat the Mausoleum version of Mom's Heart behind the flesh door, then reach Corpse II and defeat Mother. source
  - Lazarus (Character)
- Lazarus (Character) → The Beast (hard)
  1. A Strange Door is unlocked by beating Mother for the first time. source
  2. Take The Polaroid or The Negative after Mom, escape the boss room, then use it to open A Strange Door. source
  3. Find Dad's Note, complete the Ascent to Home, then defeat Dogma, the Ultra Harbingers, and The Beast. source
  - Lazarus (Character)
- Eden (Character) → Mom's Heart or It Lives! (hard)
  - Eden (Character)
- Eden (Character) → Isaac (hard)
  - Eden (Character)
- Eden (Character) → ??? (hard)
  - Eden (Character)
- Eden (Character) → Satan (hard)
  - Eden (Character)
- Eden (Character) → The Lamb (hard)
  1. Defeat Satan 5 times to unlock The Negative. source
  2. The Negative is a guaranteed drop from Mom; take it, then defeat Satan in Sheol and touch the chest to enter Dark Room. source
  3. Find and defeat The Lamb in Dark Room. source
  - Eden (Character)
- Eden (Character) → Mega Satan (hard)
  1. ??? or The Lamb must be defeated once for the Golden Gate to spawn. source
  2. Open the Golden Gate by combining Key Piece 1 and Key Piece 2 into the Golden Key, or by using Dad's Key or another gate-opening effect. source
  3. Reach the starting room of Chest or Dark Room, pass through the Golden Gate, and defeat Mega Satan. source
  - Eden (Character)
- Eden (Character) → Boss Rush (hard)
  - Eden (Character)
- Eden (Character) → Hush (hard)
  1. Defeat Mom's Heart for the 10th time to unlock Blue Womb. source
  2. Defeat Mom's Heart or It Lives! within 30 minutes and enter the door to Blue Womb. source
  3. Defeat Hush in Blue Womb. source
  - Eden (Character)
- Eden (Character) → Ultra Greedier (hard)
  - Eden (Character)
- Eden (Character) → Delirium (hard)
  1. Defeat Hush to unlock The Void. source
  2. A Void portal can appear after a major boss; defeating Hush guarantees one. source
  3. Defeat Delirium, the main boss of The Void. source
  - Eden (Character)
- Eden (Character) → Mother (hard)
  1. Defeat Hush 3 times to unlock A Secret Exit. source
  2. After A Secret Exit is unlocked, a locked door to Downpour appears after completing Basement/Cellar/Burning Basement I or II. source
  3. Collect Knife Piece 1 in Downpour/Dross II. source
  4. Collect Knife Piece 2 in Mines/Ashpit II. source
  5. After defeating Mom in Mausoleum/Gehenna II, stab the flesh door that appears with the knife familiar to open it. source
  6. Defeat the Mausoleum version of Mom's Heart behind the flesh door, then reach Corpse II and defeat Mother. source
  - Eden (Character)
- Eden (Character) → The Beast (hard)
  1. A Strange Door is unlocked by beating Mother for the first time. source
  2. Take The Polaroid or The Negative after Mom, escape the boss room, then use it to open A Strange Door. source
  3. Find Dad's Note, complete the Ascent to Home, then defeat Dogma, the Ultra Harbingers, and The Beast. source
  - Eden (Character)
- The Lost (Character) → Mom's Heart or It Lives! (hard)
  - The Lost (Character)
- The Lost (Character) → Isaac (hard)
  - The Lost (Character)
- The Lost (Character) → ??? (hard)
  - The Lost (Character)
- The Lost (Character) → Satan (hard)
  - The Lost (Character)
- The Lost (Character) → The Lamb (hard)
  1. Defeat Satan 5 times to unlock The Negative. source
  2. The Negative is a guaranteed drop from Mom; take it, then defeat Satan in Sheol and touch the chest to enter Dark Room. source
  3. Find and defeat The Lamb in Dark Room. source
  - The Lost (Character)
- The Lost (Character) → Mega Satan (hard)
  1. ??? or The Lamb must be defeated once for the Golden Gate to spawn. source
  2. Open the Golden Gate by combining Key Piece 1 and Key Piece 2 into the Golden Key, or by using Dad's Key or another gate-opening effect. source
  3. Reach the starting room of Chest or Dark Room, pass through the Golden Gate, and defeat Mega Satan. source
  - The Lost (Character)
- The Lost (Character) → Boss Rush (hard)
  - The Lost (Character)
- The Lost (Character) → Hush (hard)
  1. Defeat Mom's Heart for the 10th time to unlock Blue Womb. source
  2. Defeat Mom's Heart or It Lives! within 30 minutes and enter the door to Blue Womb. source
  3. Defeat Hush in Blue Womb. source
  - The Lost (Character)
- The Lost (Character) → Ultra Greedier (hard)
  - The Lost (Character)
- The Lost (Character) → Delirium (hard)
  1. Defeat Hush to unlock The Void. source
  2. A Void portal can appear after a major boss; defeating Hush guarantees one. source
  3. Defeat Delirium, the main boss of The Void. source
  - The Lost (Character)
- The Lost (Character) → Mother (hard)
  1. Defeat Hush 3 times to unlock A Secret Exit. source
  2. After A Secret Exit is unlocked, a locked door to Downpour appears after completing Basement/Cellar/Burning Basement I or II. source
  3. Collect Knife Piece 1 in Downpour/Dross II. source
  4. Collect Knife Piece 2 in Mines/Ashpit II. source
  5. After defeating Mom in Mausoleum/Gehenna II, stab the flesh door that appears with the knife familiar to open it. source
  6. Defeat the Mausoleum version of Mom's Heart behind the flesh door, then reach Corpse II and defeat Mother. source
  - The Lost (Character)
- The Lost (Character) → The Beast (hard)
  1. A Strange Door is unlocked by beating Mother for the first time. source
  2. Take The Polaroid or The Negative after Mom, escape the boss room, then use it to open A Strange Door. source
  3. Find Dad's Note, complete the Ascent to Home, then defeat Dogma, the Ultra Harbingers, and The Beast. source
  - The Lost (Character)
- Lilith (Character) → Mom's Heart or It Lives! (hard)
  - Lilith (Character)
- Lilith (Character) → Isaac (hard)
  - Lilith (Character)
- Lilith (Character) → ??? (hard)
  - Lilith (Character)
- Lilith (Character) → Satan (hard)
  - Lilith (Character)
- Lilith (Character) → The Lamb (hard)
  1. Defeat Satan 5 times to unlock The Negative. source
  2. The Negative is a guaranteed drop from Mom; take it, then defeat Satan in Sheol and touch the chest to enter Dark Room. source
  3. Find and defeat The Lamb in Dark Room. source
  - Lilith (Character)
- Lilith (Character) → Mega Satan (hard)
  1. ??? or The Lamb must be defeated once for the Golden Gate to spawn. source
  2. Open the Golden Gate by combining Key Piece 1 and Key Piece 2 into the Golden Key, or by using Dad's Key or another gate-opening effect. source
  3. Reach the starting room of Chest or Dark Room, pass through the Golden Gate, and defeat Mega Satan. source
  - Lilith (Character)
- Lilith (Character) → Boss Rush (hard)
  - Lilith (Character)
- Lilith (Character) → Hush (hard)
  1. Defeat Mom's Heart for the 10th time to unlock Blue Womb. source
  2. Defeat Mom's Heart or It Lives! within 30 minutes and enter the door to Blue Womb. source
  3. Defeat Hush in Blue Womb. source
  - Lilith (Character)
- Lilith (Character) → Ultra Greedier (hard)
  - Lilith (Character)
- Lilith (Character) → Delirium (hard)
  1. Defeat Hush to unlock The Void. source
  2. A Void portal can appear after a major boss; defeating Hush guarantees one. source
  3. Defeat Delirium, the main boss of The Void. source
  - Lilith (Character)
- Lilith (Character) → Mother (hard)
  1. Defeat Hush 3 times to unlock A Secret Exit. source
  2. After A Secret Exit is unlocked, a locked door to Downpour appears after completing Basement/Cellar/Burning Basement I or II. source
  3. Collect Knife Piece 1 in Downpour/Dross II. source
  4. Collect Knife Piece 2 in Mines/Ashpit II. source
  5. After defeating Mom in Mausoleum/Gehenna II, stab the flesh door that appears with the knife familiar to open it. source
  6. Defeat the Mausoleum version of Mom's Heart behind the flesh door, then reach Corpse II and defeat Mother. source
  - Lilith (Character)
- Lilith (Character) → The Beast (hard)
  1. A Strange Door is unlocked by beating Mother for the first time. source
  2. Take The Polaroid or The Negative after Mom, escape the boss room, then use it to open A Strange Door. source
  3. Find Dad's Note, complete the Ascent to Home, then defeat Dogma, the Ultra Harbingers, and The Beast. source
  - Lilith (Character)
- Keeper (Character) → Mom's Heart or It Lives! (hard)
  - Keeper (Character)
- Keeper (Character) → Isaac (hard)
  - Keeper (Character)
- Keeper (Character) → ??? (hard)
  - Keeper (Character)
- Keeper (Character) → Satan (hard)
  - Keeper (Character)
- Keeper (Character) → The Lamb (hard)
  1. Defeat Satan 5 times to unlock The Negative. source
  2. The Negative is a guaranteed drop from Mom; take it, then defeat Satan in Sheol and touch the chest to enter Dark Room. source
  3. Find and defeat The Lamb in Dark Room. source
  - Keeper (Character)
- Keeper (Character) → Mega Satan (hard)
  1. ??? or The Lamb must be defeated once for the Golden Gate to spawn. source
  2. Open the Golden Gate by combining Key Piece 1 and Key Piece 2 into the Golden Key, or by using Dad's Key or another gate-opening effect. source
  3. Reach the starting room of Chest or Dark Room, pass through the Golden Gate, and defeat Mega Satan. source
  - Keeper (Character)
- Keeper (Character) → Boss Rush (hard)
  - Keeper (Character)
- Keeper (Character) → Hush (hard)
  1. Defeat Mom's Heart for the 10th time to unlock Blue Womb. source
  2. Defeat Mom's Heart or It Lives! within 30 minutes and enter the door to Blue Womb. source
  3. Defeat Hush in Blue Womb. source
  - Keeper (Character)
- Keeper (Character) → Ultra Greedier (hard)
  - Keeper (Character)
- Keeper (Character) → Delirium (hard)
  1. Defeat Hush to unlock The Void. source
  2. A Void portal can appear after a major boss; defeating Hush guarantees one. source
  3. Defeat Delirium, the main boss of The Void. source
  - Keeper (Character)
- Keeper (Character) → Mother (hard)
  1. Defeat Hush 3 times to unlock A Secret Exit. source
  2. After A Secret Exit is unlocked, a locked door to Downpour appears after completing Basement/Cellar/Burning Basement I or II. source
  3. Collect Knife Piece 1 in Downpour/Dross II. source
  4. Collect Knife Piece 2 in Mines/Ashpit II. source
  5. After defeating Mom in Mausoleum/Gehenna II, stab the flesh door that appears with the knife familiar to open it. source
  6. Defeat the Mausoleum version of Mom's Heart behind the flesh door, then reach Corpse II and defeat Mother. source
  - Keeper (Character)
- Keeper (Character) → The Beast (hard)
  1. A Strange Door is unlocked by beating Mother for the first time. source
  2. Take The Polaroid or The Negative after Mom, escape the boss room, then use it to open A Strange Door. source
  3. Find Dad's Note, complete the Ascent to Home, then defeat Dogma, the Ultra Harbingers, and The Beast. source
  - Keeper (Character)
- Apollyon (Character) → Mom's Heart or It Lives! (hard)
  - Apollyon (Character)
- Apollyon (Character) → Isaac (hard)
  - Apollyon (Character)
- Apollyon (Character) → ??? (hard)
  - Apollyon (Character)
- Apollyon (Character) → Satan (hard)
  - Apollyon (Character)
- Apollyon (Character) → The Lamb (hard)
  1. Defeat Satan 5 times to unlock The Negative. source
  2. The Negative is a guaranteed drop from Mom; take it, then defeat Satan in Sheol and touch the chest to enter Dark Room. source
  3. Find and defeat The Lamb in Dark Room. source
  - Apollyon (Character)
- Apollyon (Character) → Mega Satan (hard)
  1. ??? or The Lamb must be defeated once for the Golden Gate to spawn. source
  2. Open the Golden Gate by combining Key Piece 1 and Key Piece 2 into the Golden Key, or by using Dad's Key or another gate-opening effect. source
  3. Reach the starting room of Chest or Dark Room, pass through the Golden Gate, and defeat Mega Satan. source
  - Apollyon (Character)
- Apollyon (Character) → Boss Rush (hard)
  - Apollyon (Character)
- Apollyon (Character) → Hush (hard)
  1. Defeat Mom's Heart for the 10th time to unlock Blue Womb. source
  2. Defeat Mom's Heart or It Lives! within 30 minutes and enter the door to Blue Womb. source
  3. Defeat Hush in Blue Womb. source
  - Apollyon (Character)
- Apollyon (Character) → Ultra Greedier (hard)
  - Apollyon (Character)
- Apollyon (Character) → Delirium (hard)
  1. Defeat Hush to unlock The Void. source
  2. A Void portal can appear after a major boss; defeating Hush guarantees one. source
  3. Defeat Delirium, the main boss of The Void. source
  - Apollyon (Character)
- Apollyon (Character) → Mother (hard)
  1. Defeat Hush 3 times to unlock A Secret Exit. source
  2. After A Secret Exit is unlocked, a locked door to Downpour appears after completing Basement/Cellar/Burning Basement I or II. source
  3. Collect Knife Piece 1 in Downpour/Dross II. source
  4. Collect Knife Piece 2 in Mines/Ashpit II. source
  5. After defeating Mom in Mausoleum/Gehenna II, stab the flesh door that appears with the knife familiar to open it. source
  6. Defeat the Mausoleum version of Mom's Heart behind the flesh door, then reach Corpse II and defeat Mother. source
  - Apollyon (Character)
- Apollyon (Character) → The Beast (hard)
  1. A Strange Door is unlocked by beating Mother for the first time. source
  2. Take The Polaroid or The Negative after Mom, escape the boss room, then use it to open A Strange Door. source
  3. Find Dad's Note, complete the Ascent to Home, then defeat Dogma, the Ultra Harbingers, and The Beast. source
  - Apollyon (Character)
- The Forgotten (Character) → Mom's Heart or It Lives! (hard)
  - The Forgotten (Character)
- The Forgotten (Character) → Isaac (hard)
  - The Forgotten (Character)
- The Forgotten (Character) → ??? (hard)
  - The Forgotten (Character)
- The Forgotten (Character) → Satan (hard)
  - The Forgotten (Character)
- The Forgotten (Character) → The Lamb (hard)
  1. Defeat Satan 5 times to unlock The Negative. source
  2. The Negative is a guaranteed drop from Mom; take it, then defeat Satan in Sheol and touch the chest to enter Dark Room. source
  3. Find and defeat The Lamb in Dark Room. source
  - The Forgotten (Character)
- The Forgotten (Character) → Mega Satan (hard)
  1. ??? or The Lamb must be defeated once for the Golden Gate to spawn. source
  2. Open the Golden Gate by combining Key Piece 1 and Key Piece 2 into the Golden Key, or by using Dad's Key or another gate-opening effect. source
  3. Reach the starting room of Chest or Dark Room, pass through the Golden Gate, and defeat Mega Satan. source
  - The Forgotten (Character)
- The Forgotten (Character) → Boss Rush (hard)
  - The Forgotten (Character)
- The Forgotten (Character) → Hush (hard)
  1. Defeat Mom's Heart for the 10th time to unlock Blue Womb. source
  2. Defeat Mom's Heart or It Lives! within 30 minutes and enter the door to Blue Womb. source
  3. Defeat Hush in Blue Womb. source
  - The Forgotten (Character)
- The Forgotten (Character) → Ultra Greedier (hard)
  - The Forgotten (Character)
- The Forgotten (Character) → Delirium (hard)
  1. Defeat Hush to unlock The Void. source
  2. A Void portal can appear after a major boss; defeating Hush guarantees one. source
  3. Defeat Delirium, the main boss of The Void. source
  - The Forgotten (Character)
- The Forgotten (Character) → Mother (hard)
  1. Defeat Hush 3 times to unlock A Secret Exit. source
  2. After A Secret Exit is unlocked, a locked door to Downpour appears after completing Basement/Cellar/Burning Basement I or II. source
  3. Collect Knife Piece 1 in Downpour/Dross II. source
  4. Collect Knife Piece 2 in Mines/Ashpit II. source
  5. After defeating Mom in Mausoleum/Gehenna II, stab the flesh door that appears with the knife familiar to open it. source
  6. Defeat the Mausoleum version of Mom's Heart behind the flesh door, then reach Corpse II and defeat Mother. source
  - The Forgotten (Character)
- The Forgotten (Character) → The Beast (hard)
  1. A Strange Door is unlocked by beating Mother for the first time. source
  2. Take The Polaroid or The Negative after Mom, escape the boss room, then use it to open A Strange Door. source
  3. Find Dad's Note, complete the Ascent to Home, then defeat Dogma, the Ultra Harbingers, and The Beast. source
  - The Forgotten (Character)
- Bethany (Character) → Mom's Heart or It Lives! (hard)
  - Bethany (Character)
- Bethany (Character) → Isaac (hard)
  - Bethany (Character)
- Bethany (Character) → ??? (hard)
  - Bethany (Character)
- Bethany (Character) → Satan (hard)
  - Bethany (Character)
- Bethany (Character) → The Lamb (hard)
  1. Defeat Satan 5 times to unlock The Negative. source
  2. The Negative is a guaranteed drop from Mom; take it, then defeat Satan in Sheol and touch the chest to enter Dark Room. source
  3. Find and defeat The Lamb in Dark Room. source
  - Bethany (Character)
- Bethany (Character) → Mega Satan (hard)
  1. ??? or The Lamb must be defeated once for the Golden Gate to spawn. source
  2. Open the Golden Gate by combining Key Piece 1 and Key Piece 2 into the Golden Key, or by using Dad's Key or another gate-opening effect. source
  3. Reach the starting room of Chest or Dark Room, pass through the Golden Gate, and defeat Mega Satan. source
  - Bethany (Character)
- Bethany (Character) → Boss Rush (hard)
  - Bethany (Character)
- Bethany (Character) → Hush (hard)
  1. Defeat Mom's Heart for the 10th time to unlock Blue Womb. source
  2. Defeat Mom's Heart or It Lives! within 30 minutes and enter the door to Blue Womb. source
  3. Defeat Hush in Blue Womb. source
  - Bethany (Character)
- Bethany (Character) → Ultra Greedier (hard)
  - Bethany (Character)
- Bethany (Character) → Delirium (hard)
  1. Defeat Hush to unlock The Void. source
  2. A Void portal can appear after a major boss; defeating Hush guarantees one. source
  3. Defeat Delirium, the main boss of The Void. source
  - Bethany (Character)
- Bethany (Character) → Mother (hard)
  1. Defeat Hush 3 times to unlock A Secret Exit. source
  2. After A Secret Exit is unlocked, a locked door to Downpour appears after completing Basement/Cellar/Burning Basement I or II. source
  3. Collect Knife Piece 1 in Downpour/Dross II. source
  4. Collect Knife Piece 2 in Mines/Ashpit II. source
  5. After defeating Mom in Mausoleum/Gehenna II, stab the flesh door that appears with the knife familiar to open it. source
  6. Defeat the Mausoleum version of Mom's Heart behind the flesh door, then reach Corpse II and defeat Mother. source
  - Bethany (Character)
- Bethany (Character) → The Beast (hard)
  1. A Strange Door is unlocked by beating Mother for the first time. source
  2. Take The Polaroid or The Negative after Mom, escape the boss room, then use it to open A Strange Door. source
  3. Find Dad's Note, complete the Ascent to Home, then defeat Dogma, the Ultra Harbingers, and The Beast. source
  - Bethany (Character)
- Jacob & Esau → Mom's Heart or It Lives! (hard)
  - Jacob & Esau
- Jacob & Esau → Isaac (hard)
  - Jacob & Esau
- Jacob & Esau → ??? (hard)
  - Jacob & Esau
- Jacob & Esau → Satan (hard)
  - Jacob & Esau
- Jacob & Esau → The Lamb (hard)
  1. Defeat Satan 5 times to unlock The Negative. source
  2. The Negative is a guaranteed drop from Mom; take it, then defeat Satan in Sheol and touch the chest to enter Dark Room. source
  3. Find and defeat The Lamb in Dark Room. source
  - Jacob & Esau
- Jacob & Esau → Mega Satan (hard)
  1. ??? or The Lamb must be defeated once for the Golden Gate to spawn. source
  2. Open the Golden Gate by combining Key Piece 1 and Key Piece 2 into the Golden Key, or by using Dad's Key or another gate-opening effect. source
  3. Reach the starting room of Chest or Dark Room, pass through the Golden Gate, and defeat Mega Satan. source
  - Jacob & Esau
- Jacob & Esau → Boss Rush (hard)
  - Jacob & Esau
- Jacob & Esau → Hush (hard)
  1. Defeat Mom's Heart for the 10th time to unlock Blue Womb. source
  2. Defeat Mom's Heart or It Lives! within 30 minutes and enter the door to Blue Womb. source
  3. Defeat Hush in Blue Womb. source
  - Jacob & Esau
- Jacob & Esau → Ultra Greedier (hard)
  - Jacob & Esau
- Jacob & Esau → Delirium (hard)
  1. Defeat Hush to unlock The Void. source
  2. A Void portal can appear after a major boss; defeating Hush guarantees one. source
  3. Defeat Delirium, the main boss of The Void. source
  - Jacob & Esau
- Jacob & Esau → Mother (hard)
  1. Defeat Hush 3 times to unlock A Secret Exit. source
  2. After A Secret Exit is unlocked, a locked door to Downpour appears after completing Basement/Cellar/Burning Basement I or II. source
  3. Collect Knife Piece 1 in Downpour/Dross II. source
  4. Collect Knife Piece 2 in Mines/Ashpit II. source
  5. After defeating Mom in Mausoleum/Gehenna II, stab the flesh door that appears with the knife familiar to open it. source
  6. Defeat the Mausoleum version of Mom's Heart behind the flesh door, then reach Corpse II and defeat Mother. source
  - Jacob & Esau
- Jacob & Esau → The Beast (hard)
  1. A Strange Door is unlocked by beating Mother for the first time. source
  2. Take The Polaroid or The Negative after Mom, escape the boss room, then use it to open A Strange Door. source
  3. Find Dad's Note, complete the Ascent to Home, then defeat Dogma, the Ultra Harbingers, and The Beast. source
  - Jacob & Esau
- Tainted Isaac → Mom's Heart or It Lives! (hard)
  - Tainted Isaac
- Tainted Isaac → Isaac (hard)
  - Tainted Isaac
- Tainted Isaac → ??? (hard)
  - Tainted Isaac
- Tainted Isaac → Satan (hard)
  - Tainted Isaac
- Tainted Isaac → The Lamb (hard)
  1. Defeat Satan 5 times to unlock The Negative. source
  2. The Negative is a guaranteed drop from Mom; take it, then defeat Satan in Sheol and touch the chest to enter Dark Room. source
  3. Find and defeat The Lamb in Dark Room. source
  - Tainted Isaac
- Tainted Isaac → Mega Satan (hard)
  1. ??? or The Lamb must be defeated once for the Golden Gate to spawn. source
  2. Open the Golden Gate by combining Key Piece 1 and Key Piece 2 into the Golden Key, or by using Dad's Key or another gate-opening effect. source
  3. Reach the starting room of Chest or Dark Room, pass through the Golden Gate, and defeat Mega Satan. source
  - Tainted Isaac
- Tainted Isaac → Boss Rush (hard)
  - Tainted Isaac
- Tainted Isaac → Hush (hard)
  1. Defeat Mom's Heart for the 10th time to unlock Blue Womb. source
  2. Defeat Mom's Heart or It Lives! within 30 minutes and enter the door to Blue Womb. source
  3. Defeat Hush in Blue Womb. source
  - Tainted Isaac
- Tainted Isaac → Ultra Greedier (hard)
  - Tainted Isaac
- Tainted Isaac → Delirium (hard)
  1. Defeat Hush to unlock The Void. source
  2. A Void portal can appear after a major boss; defeating Hush guarantees one. source
  3. Defeat Delirium, the main boss of The Void. source
  - Tainted Isaac
- Tainted Isaac → Mother (hard)
  1. Defeat Hush 3 times to unlock A Secret Exit. source
  2. After A Secret Exit is unlocked, a locked door to Downpour appears after completing Basement/Cellar/Burning Basement I or II. source
  3. Collect Knife Piece 1 in Downpour/Dross II. source
  4. Collect Knife Piece 2 in Mines/Ashpit II. source
  5. After defeating Mom in Mausoleum/Gehenna II, stab the flesh door that appears with the knife familiar to open it. source
  6. Defeat the Mausoleum version of Mom's Heart behind the flesh door, then reach Corpse II and defeat Mother. source
  - Tainted Isaac
- Tainted Isaac → The Beast (hard)
  1. A Strange Door is unlocked by beating Mother for the first time. source
  2. Take The Polaroid or The Negative after Mom, escape the boss room, then use it to open A Strange Door. source
  3. Find Dad's Note, complete the Ascent to Home, then defeat Dogma, the Ultra Harbingers, and The Beast. source
  - Tainted Isaac
- Tainted Magdalene → Mom's Heart or It Lives! (hard)
  - Tainted Magdalene
- Tainted Magdalene → Isaac (hard)
  - Tainted Magdalene
- Tainted Magdalene → ??? (hard)
  - Tainted Magdalene
- Tainted Magdalene → Satan (hard)
  - Tainted Magdalene
- Tainted Magdalene → The Lamb (hard)
  1. Defeat Satan 5 times to unlock The Negative. source
  2. The Negative is a guaranteed drop from Mom; take it, then defeat Satan in Sheol and touch the chest to enter Dark Room. source
  3. Find and defeat The Lamb in Dark Room. source
  - Tainted Magdalene
- Tainted Magdalene → Mega Satan (hard)
  1. ??? or The Lamb must be defeated once for the Golden Gate to spawn. source
  2. Open the Golden Gate by combining Key Piece 1 and Key Piece 2 into the Golden Key, or by using Dad's Key or another gate-opening effect. source
  3. Reach the starting room of Chest or Dark Room, pass through the Golden Gate, and defeat Mega Satan. source
  - Tainted Magdalene
- Tainted Magdalene → Boss Rush (hard)
  - Tainted Magdalene
- Tainted Magdalene → Hush (hard)
  1. Defeat Mom's Heart for the 10th time to unlock Blue Womb. source
  2. Defeat Mom's Heart or It Lives! within 30 minutes and enter the door to Blue Womb. source
  3. Defeat Hush in Blue Womb. source
  - Tainted Magdalene
- Tainted Magdalene → Ultra Greedier (hard)
  - Tainted Magdalene
- Tainted Magdalene → Delirium (hard)
  1. Defeat Hush to unlock The Void. source
  2. A Void portal can appear after a major boss; defeating Hush guarantees one. source
  3. Defeat Delirium, the main boss of The Void. source
  - Tainted Magdalene
- Tainted Magdalene → Mother (hard)
  1. Defeat Hush 3 times to unlock A Secret Exit. source
  2. After A Secret Exit is unlocked, a locked door to Downpour appears after completing Basement/Cellar/Burning Basement I or II. source
  3. Collect Knife Piece 1 in Downpour/Dross II. source
  4. Collect Knife Piece 2 in Mines/Ashpit II. source
  5. After defeating Mom in Mausoleum/Gehenna II, stab the flesh door that appears with the knife familiar to open it. source
  6. Defeat the Mausoleum version of Mom's Heart behind the flesh door, then reach Corpse II and defeat Mother. source
  - Tainted Magdalene
- Tainted Magdalene → The Beast (hard)
  1. A Strange Door is unlocked by beating Mother for the first time. source
  2. Take The Polaroid or The Negative after Mom, escape the boss room, then use it to open A Strange Door. source
  3. Find Dad's Note, complete the Ascent to Home, then defeat Dogma, the Ultra Harbingers, and The Beast. source
  - Tainted Magdalene
- Tainted Cain → Mom's Heart or It Lives! (hard)
  - Tainted Cain
- Tainted Cain → Isaac (hard)
  - Tainted Cain
- Tainted Cain → ??? (hard)
  - Tainted Cain
- Tainted Cain → Satan (hard)
  - Tainted Cain
- Tainted Cain → The Lamb (hard)
  1. Defeat Satan 5 times to unlock The Negative. source
  2. The Negative is a guaranteed drop from Mom; take it, then defeat Satan in Sheol and touch the chest to enter Dark Room. source
  3. Find and defeat The Lamb in Dark Room. source
  - Tainted Cain
- Tainted Cain → Mega Satan (hard)
  1. ??? or The Lamb must be defeated once for the Golden Gate to spawn. source
  2. Open the Golden Gate by combining Key Piece 1 and Key Piece 2 into the Golden Key, or by using Dad's Key or another gate-opening effect. source
  3. Reach the starting room of Chest or Dark Room, pass through the Golden Gate, and defeat Mega Satan. source
  - Tainted Cain
- Tainted Cain → Boss Rush (hard)
  - Tainted Cain
- Tainted Cain → Hush (hard)
  1. Defeat Mom's Heart for the 10th time to unlock Blue Womb. source
  2. Defeat Mom's Heart or It Lives! within 30 minutes and enter the door to Blue Womb. source
  3. Defeat Hush in Blue Womb. source
  - Tainted Cain
- Tainted Cain → Ultra Greedier (hard)
  - Tainted Cain
- Tainted Cain → Delirium (hard)
  1. Defeat Hush to unlock The Void. source
  2. A Void portal can appear after a major boss; defeating Hush guarantees one. source
  3. Defeat Delirium, the main boss of The Void. source
  - Tainted Cain
- Tainted Cain → Mother (hard)
  1. Defeat Hush 3 times to unlock A Secret Exit. source
  2. After A Secret Exit is unlocked, a locked door to Downpour appears after completing Basement/Cellar/Burning Basement I or II. source
  3. Collect Knife Piece 1 in Downpour/Dross II. source
  4. Collect Knife Piece 2 in Mines/Ashpit II. source
  5. After defeating Mom in Mausoleum/Gehenna II, stab the flesh door that appears with the knife familiar to open it. source
  6. Defeat the Mausoleum version of Mom's Heart behind the flesh door, then reach Corpse II and defeat Mother. source
  - Tainted Cain
- Tainted Cain → The Beast (hard)
  1. A Strange Door is unlocked by beating Mother for the first time. source
  2. Take The Polaroid or The Negative after Mom, escape the boss room, then use it to open A Strange Door. source
  3. Find Dad's Note, complete the Ascent to Home, then defeat Dogma, the Ultra Harbingers, and The Beast. source
  - Tainted Cain
- Tainted Judas → Mom's Heart or It Lives! (hard)
  - Tainted Judas
- Tainted Judas → Isaac (hard)
  - Tainted Judas
- Tainted Judas → ??? (hard)
  - Tainted Judas
- Tainted Judas → Satan (hard)
  - Tainted Judas
- Tainted Judas → The Lamb (hard)
  1. Defeat Satan 5 times to unlock The Negative. source
  2. The Negative is a guaranteed drop from Mom; take it, then defeat Satan in Sheol and touch the chest to enter Dark Room. source
  3. Find and defeat The Lamb in Dark Room. source
  - Tainted Judas
- Tainted Judas → Mega Satan (hard)
  1. ??? or The Lamb must be defeated once for the Golden Gate to spawn. source
  2. Open the Golden Gate by combining Key Piece 1 and Key Piece 2 into the Golden Key, or by using Dad's Key or another gate-opening effect. source
  3. Reach the starting room of Chest or Dark Room, pass through the Golden Gate, and defeat Mega Satan. source
  - Tainted Judas
- Tainted Judas → Boss Rush (hard)
  - Tainted Judas
- Tainted Judas → Hush (hard)
  1. Defeat Mom's Heart for the 10th time to unlock Blue Womb. source
  2. Defeat Mom's Heart or It Lives! within 30 minutes and enter the door to Blue Womb. source
  3. Defeat Hush in Blue Womb. source
  - Tainted Judas
- Tainted Judas → Ultra Greedier (hard)
  - Tainted Judas
- Tainted Judas → Delirium (hard)
  1. Defeat Hush to unlock The Void. source
  2. A Void portal can appear after a major boss; defeating Hush guarantees one. source
  3. Defeat Delirium, the main boss of The Void. source
  - Tainted Judas
- Tainted Judas → Mother (hard)
  1. Defeat Hush 3 times to unlock A Secret Exit. source
  2. After A Secret Exit is unlocked, a locked door to Downpour appears after completing Basement/Cellar/Burning Basement I or II. source
  3. Collect Knife Piece 1 in Downpour/Dross II. source
  4. Collect Knife Piece 2 in Mines/Ashpit II. source
  5. After defeating Mom in Mausoleum/Gehenna II, stab the flesh door that appears with the knife familiar to open it. source
  6. Defeat the Mausoleum version of Mom's Heart behind the flesh door, then reach Corpse II and defeat Mother. source
  - Tainted Judas
- Tainted Judas → The Beast (hard)
  1. A Strange Door is unlocked by beating Mother for the first time. source
  2. Take The Polaroid or The Negative after Mom, escape the boss room, then use it to open A Strange Door. source
  3. Find Dad's Note, complete the Ascent to Home, then defeat Dogma, the Ultra Harbingers, and The Beast. source
  - Tainted Judas
- Tainted ??? → Mom's Heart or It Lives! (hard)
  - Tainted ???
- Tainted ??? → Isaac (hard)
  - Tainted ???
- Tainted ??? → ??? (hard)
  - Tainted ???
- Tainted ??? → Satan (hard)
  - Tainted ???
- Tainted ??? → The Lamb (hard)
  1. Defeat Satan 5 times to unlock The Negative. source
  2. The Negative is a guaranteed drop from Mom; take it, then defeat Satan in Sheol and touch the chest to enter Dark Room. source
  3. Find and defeat The Lamb in Dark Room. source
  - Tainted ???
- Tainted ??? → Mega Satan (hard)
  1. ??? or The Lamb must be defeated once for the Golden Gate to spawn. source
  2. Open the Golden Gate by combining Key Piece 1 and Key Piece 2 into the Golden Key, or by using Dad's Key or another gate-opening effect. source
  3. Reach the starting room of Chest or Dark Room, pass through the Golden Gate, and defeat Mega Satan. source
  - Tainted ???
- Tainted ??? → Boss Rush (hard)
  - Tainted ???
- Tainted ??? → Hush (hard)
  1. Defeat Mom's Heart for the 10th time to unlock Blue Womb. source
  2. Defeat Mom's Heart or It Lives! within 30 minutes and enter the door to Blue Womb. source
  3. Defeat Hush in Blue Womb. source
  - Tainted ???
- Tainted ??? → Ultra Greedier (hard)
  - Tainted ???
- Tainted ??? → Delirium (hard)
  1. Defeat Hush to unlock The Void. source
  2. A Void portal can appear after a major boss; defeating Hush guarantees one. source
  3. Defeat Delirium, the main boss of The Void. source
  - Tainted ???
- Tainted ??? → Mother (hard)
  1. Defeat Hush 3 times to unlock A Secret Exit. source
  2. After A Secret Exit is unlocked, a locked door to Downpour appears after completing Basement/Cellar/Burning Basement I or II. source
  3. Collect Knife Piece 1 in Downpour/Dross II. source
  4. Collect Knife Piece 2 in Mines/Ashpit II. source
  5. After defeating Mom in Mausoleum/Gehenna II, stab the flesh door that appears with the knife familiar to open it. source
  6. Defeat the Mausoleum version of Mom's Heart behind the flesh door, then reach Corpse II and defeat Mother. source
  - Tainted ???
- Tainted ??? → The Beast (hard)
  1. A Strange Door is unlocked by beating Mother for the first time. source
  2. Take The Polaroid or The Negative after Mom, escape the boss room, then use it to open A Strange Door. source
  3. Find Dad's Note, complete the Ascent to Home, then defeat Dogma, the Ultra Harbingers, and The Beast. source
  - Tainted ???
- Tainted Eve → Mom's Heart or It Lives! (hard)
  - Tainted Eve
- Tainted Eve → Isaac (hard)
  - Tainted Eve
- Tainted Eve → ??? (hard)
  - Tainted Eve
- Tainted Eve → Satan (hard)
  - Tainted Eve
- Tainted Eve → The Lamb (hard)
  1. Defeat Satan 5 times to unlock The Negative. source
  2. The Negative is a guaranteed drop from Mom; take it, then defeat Satan in Sheol and touch the chest to enter Dark Room. source
  3. Find and defeat The Lamb in Dark Room. source
  - Tainted Eve
- Tainted Eve → Mega Satan (hard)
  1. ??? or The Lamb must be defeated once for the Golden Gate to spawn. source
  2. Open the Golden Gate by combining Key Piece 1 and Key Piece 2 into the Golden Key, or by using Dad's Key or another gate-opening effect. source
  3. Reach the starting room of Chest or Dark Room, pass through the Golden Gate, and defeat Mega Satan. source
  - Tainted Eve
- Tainted Eve → Boss Rush (hard)
  - Tainted Eve
- Tainted Eve → Hush (hard)
  1. Defeat Mom's Heart for the 10th time to unlock Blue Womb. source
  2. Defeat Mom's Heart or It Lives! within 30 minutes and enter the door to Blue Womb. source
  3. Defeat Hush in Blue Womb. source
  - Tainted Eve
- Tainted Eve → Ultra Greedier (hard)
  - Tainted Eve
- Tainted Eve → Delirium (hard)
  1. Defeat Hush to unlock The Void. source
  2. A Void portal can appear after a major boss; defeating Hush guarantees one. source
  3. Defeat Delirium, the main boss of The Void. source
  - Tainted Eve
- Tainted Eve → Mother (hard)
  1. Defeat Hush 3 times to unlock A Secret Exit. source
  2. After A Secret Exit is unlocked, a locked door to Downpour appears after completing Basement/Cellar/Burning Basement I or II. source
  3. Collect Knife Piece 1 in Downpour/Dross II. source
  4. Collect Knife Piece 2 in Mines/Ashpit II. source
  5. After defeating Mom in Mausoleum/Gehenna II, stab the flesh door that appears with the knife familiar to open it. source
  6. Defeat the Mausoleum version of Mom's Heart behind the flesh door, then reach Corpse II and defeat Mother. source
  - Tainted Eve
- Tainted Eve → The Beast (hard)
  1. A Strange Door is unlocked by beating Mother for the first time. source
  2. Take The Polaroid or The Negative after Mom, escape the boss room, then use it to open A Strange Door. source
  3. Find Dad's Note, complete the Ascent to Home, then defeat Dogma, the Ultra Harbingers, and The Beast. source
  - Tainted Eve
- Tainted Samson → Mom's Heart or It Lives! (hard)
  - Tainted Samson
- Tainted Samson → Isaac (hard)
  - Tainted Samson
- Tainted Samson → ??? (hard)
  - Tainted Samson
- Tainted Samson → Satan (hard)
  - Tainted Samson
- Tainted Samson → The Lamb (hard)
  1. Defeat Satan 5 times to unlock The Negative. source
  2. The Negative is a guaranteed drop from Mom; take it, then defeat Satan in Sheol and touch the chest to enter Dark Room. source
  3. Find and defeat The Lamb in Dark Room. source
  - Tainted Samson
- Tainted Samson → Mega Satan (hard)
  1. ??? or The Lamb must be defeated once for the Golden Gate to spawn. source
  2. Open the Golden Gate by combining Key Piece 1 and Key Piece 2 into the Golden Key, or by using Dad's Key or another gate-opening effect. source
  3. Reach the starting room of Chest or Dark Room, pass through the Golden Gate, and defeat Mega Satan. source
  - Tainted Samson
- Tainted Samson → Boss Rush (hard)
  - Tainted Samson
- Tainted Samson → Hush (hard)
  1. Defeat Mom's Heart for the 10th time to unlock Blue Womb. source
  2. Defeat Mom's Heart or It Lives! within 30 minutes and enter the door to Blue Womb. source
  3. Defeat Hush in Blue Womb. source
  - Tainted Samson
- Tainted Samson → Ultra Greedier (hard)
  - Tainted Samson
- Tainted Samson → Delirium (hard)
  1. Defeat Hush to unlock The Void. source
  2. A Void portal can appear after a major boss; defeating Hush guarantees one. source
  3. Defeat Delirium, the main boss of The Void. source
  - Tainted Samson
- Tainted Samson → Mother (hard)
  1. Defeat Hush 3 times to unlock A Secret Exit. source
  2. After A Secret Exit is unlocked, a locked door to Downpour appears after completing Basement/Cellar/Burning Basement I or II. source
  3. Collect Knife Piece 1 in Downpour/Dross II. source
  4. Collect Knife Piece 2 in Mines/Ashpit II. source
  5. After defeating Mom in Mausoleum/Gehenna II, stab the flesh door that appears with the knife familiar to open it. source
  6. Defeat the Mausoleum version of Mom's Heart behind the flesh door, then reach Corpse II and defeat Mother. source
  - Tainted Samson
- Tainted Samson → The Beast (hard)
  1. A Strange Door is unlocked by beating Mother for the first time. source
  2. Take The Polaroid or The Negative after Mom, escape the boss room, then use it to open A Strange Door. source
  3. Find Dad's Note, complete the Ascent to Home, then defeat Dogma, the Ultra Harbingers, and The Beast. source
  - Tainted Samson
- Tainted Azazel → Mom's Heart or It Lives! (hard)
  - Tainted Azazel
- Tainted Azazel → Isaac (hard)
  - Tainted Azazel
- Tainted Azazel → ??? (hard)
  - Tainted Azazel
- Tainted Azazel → Satan (hard)
  - Tainted Azazel
- Tainted Azazel → The Lamb (hard)
  1. Defeat Satan 5 times to unlock The Negative. source
  2. The Negative is a guaranteed drop from Mom; take it, then defeat Satan in Sheol and touch the chest to enter Dark Room. source
  3. Find and defeat The Lamb in Dark Room. source
  - Tainted Azazel
- Tainted Azazel → Mega Satan (hard)
  1. ??? or The Lamb must be defeated once for the Golden Gate to spawn. source
  2. Open the Golden Gate by combining Key Piece 1 and Key Piece 2 into the Golden Key, or by using Dad's Key or another gate-opening effect. source
  3. Reach the starting room of Chest or Dark Room, pass through the Golden Gate, and defeat Mega Satan. source
  - Tainted Azazel
- Tainted Azazel → Boss Rush (hard)
  - Tainted Azazel
- Tainted Azazel → Hush (hard)
  1. Defeat Mom's Heart for the 10th time to unlock Blue Womb. source
  2. Defeat Mom's Heart or It Lives! within 30 minutes and enter the door to Blue Womb. source
  3. Defeat Hush in Blue Womb. source
  - Tainted Azazel
- Tainted Azazel → Ultra Greedier (hard)
  - Tainted Azazel
- Tainted Azazel → Delirium (hard)
  1. Defeat Hush to unlock The Void. source
  2. A Void portal can appear after a major boss; defeating Hush guarantees one. source
  3. Defeat Delirium, the main boss of The Void. source
  - Tainted Azazel
- Tainted Azazel → Mother (hard)
  1. Defeat Hush 3 times to unlock A Secret Exit. source
  2. After A Secret Exit is unlocked, a locked door to Downpour appears after completing Basement/Cellar/Burning Basement I or II. source
  3. Collect Knife Piece 1 in Downpour/Dross II. source
  4. Collect Knife Piece 2 in Mines/Ashpit II. source
  5. After defeating Mom in Mausoleum/Gehenna II, stab the flesh door that appears with the knife familiar to open it. source
  6. Defeat the Mausoleum version of Mom's Heart behind the flesh door, then reach Corpse II and defeat Mother. source
  - Tainted Azazel
- Tainted Azazel → The Beast (hard)
  1. A Strange Door is unlocked by beating Mother for the first time. source
  2. Take The Polaroid or The Negative after Mom, escape the boss room, then use it to open A Strange Door. source
  3. Find Dad's Note, complete the Ascent to Home, then defeat Dogma, the Ultra Harbingers, and The Beast. source
  - Tainted Azazel
- Tainted Lazarus → Mom's Heart or It Lives! (hard)
  - Tainted Lazarus
- Tainted Lazarus → Isaac (hard)
  - Tainted Lazarus
- Tainted Lazarus → ??? (hard)
  - Tainted Lazarus
- Tainted Lazarus → Satan (hard)
  - Tainted Lazarus
- Tainted Lazarus → The Lamb (hard)
  1. Defeat Satan 5 times to unlock The Negative. source
  2. The Negative is a guaranteed drop from Mom; take it, then defeat Satan in Sheol and touch the chest to enter Dark Room. source
  3. Find and defeat The Lamb in Dark Room. source
  - Tainted Lazarus
- Tainted Lazarus → Mega Satan (hard)
  1. ??? or The Lamb must be defeated once for the Golden Gate to spawn. source
  2. Open the Golden Gate by combining Key Piece 1 and Key Piece 2 into the Golden Key, or by using Dad's Key or another gate-opening effect. source
  3. Reach the starting room of Chest or Dark Room, pass through the Golden Gate, and defeat Mega Satan. source
  - Tainted Lazarus
- Tainted Lazarus → Boss Rush (hard)
  - Tainted Lazarus
- Tainted Lazarus → Hush (hard)
  1. Defeat Mom's Heart for the 10th time to unlock Blue Womb. source
  2. Defeat Mom's Heart or It Lives! within 30 minutes and enter the door to Blue Womb. source
  3. Defeat Hush in Blue Womb. source
  - Tainted Lazarus
- Tainted Lazarus → Ultra Greedier (hard)
  - Tainted Lazarus
- Tainted Lazarus → Delirium (hard)
  1. Defeat Hush to unlock The Void. source
  2. A Void portal can appear after a major boss; defeating Hush guarantees one. source
  3. Defeat Delirium, the main boss of The Void. source
  - Tainted Lazarus
- Tainted Lazarus → Mother (hard)
  1. Defeat Hush 3 times to unlock A Secret Exit. source
  2. After A Secret Exit is unlocked, a locked door to Downpour appears after completing Basement/Cellar/Burning Basement I or II. source
  3. Collect Knife Piece 1 in Downpour/Dross II. source
  4. Collect Knife Piece 2 in Mines/Ashpit II. source
  5. After defeating Mom in Mausoleum/Gehenna II, stab the flesh door that appears with the knife familiar to open it. source
  6. Defeat the Mausoleum version of Mom's Heart behind the flesh door, then reach Corpse II and defeat Mother. source
  - Tainted Lazarus
- Tainted Lazarus → The Beast (hard)
  1. A Strange Door is unlocked by beating Mother for the first time. source
  2. Take The Polaroid or The Negative after Mom, escape the boss room, then use it to open A Strange Door. source
  3. Find Dad's Note, complete the Ascent to Home, then defeat Dogma, the Ultra Harbingers, and The Beast. source
  - Tainted Lazarus
- Tainted Eden → Mom's Heart or It Lives! (hard)
  - Tainted Eden
- Tainted Eden → Isaac (hard)
  - Tainted Eden
- Tainted Eden → ??? (hard)
  - Tainted Eden
- Tainted Eden → Satan (hard)
  - Tainted Eden
- Tainted Eden → The Lamb (hard)
  1. Defeat Satan 5 times to unlock The Negative. source
  2. The Negative is a guaranteed drop from Mom; take it, then defeat Satan in Sheol and touch the chest to enter Dark Room. source
  3. Find and defeat The Lamb in Dark Room. source
  - Tainted Eden
- Tainted Eden → Mega Satan (hard)
  1. ??? or The Lamb must be defeated once for the Golden Gate to spawn. source
  2. Open the Golden Gate by combining Key Piece 1 and Key Piece 2 into the Golden Key, or by using Dad's Key or another gate-opening effect. source
  3. Reach the starting room of Chest or Dark Room, pass through the Golden Gate, and defeat Mega Satan. source
  - Tainted Eden
- Tainted Eden → Boss Rush (hard)
  - Tainted Eden
- Tainted Eden → Hush (hard)
  1. Defeat Mom's Heart for the 10th time to unlock Blue Womb. source
  2. Defeat Mom's Heart or It Lives! within 30 minutes and enter the door to Blue Womb. source
  3. Defeat Hush in Blue Womb. source
  - Tainted Eden
- Tainted Eden → Ultra Greedier (hard)
  - Tainted Eden
- Tainted Eden → Delirium (hard)
  1. Defeat Hush to unlock The Void. source
  2. A Void portal can appear after a major boss; defeating Hush guarantees one. source
  3. Defeat Delirium, the main boss of The Void. source
  - Tainted Eden
- Tainted Eden → Mother (hard)
  1. Defeat Hush 3 times to unlock A Secret Exit. source
  2. After A Secret Exit is unlocked, a locked door to Downpour appears after completing Basement/Cellar/Burning Basement I or II. source
  3. Collect Knife Piece 1 in Downpour/Dross II. source
  4. Collect Knife Piece 2 in Mines/Ashpit II. source
  5. After defeating Mom in Mausoleum/Gehenna II, stab the flesh door that appears with the knife familiar to open it. source
  6. Defeat the Mausoleum version of Mom's Heart behind the flesh door, then reach Corpse II and defeat Mother. source
  - Tainted Eden
- Tainted Eden → The Beast (hard)
  1. A Strange Door is unlocked by beating Mother for the first time. source
  2. Take The Polaroid or The Negative after Mom, escape the boss room, then use it to open A Strange Door. source
  3. Find Dad's Note, complete the Ascent to Home, then defeat Dogma, the Ultra Harbingers, and The Beast. source
  - Tainted Eden
- Tainted Lost → Mom's Heart or It Lives! (hard)
  - Tainted Lost
- Tainted Lost → Isaac (hard)
  - Tainted Lost
- Tainted Lost → ??? (hard)
  - Tainted Lost
- Tainted Lost → Satan (hard)
  - Tainted Lost
- Tainted Lost → The Lamb (hard)
  1. Defeat Satan 5 times to unlock The Negative. source
  2. The Negative is a guaranteed drop from Mom; take it, then defeat Satan in Sheol and touch the chest to enter Dark Room. source
  3. Find and defeat The Lamb in Dark Room. source
  - Tainted Lost
- Tainted Lost → Mega Satan (hard)
  1. ??? or The Lamb must be defeated once for the Golden Gate to spawn. source
  2. Open the Golden Gate by combining Key Piece 1 and Key Piece 2 into the Golden Key, or by using Dad's Key or another gate-opening effect. source
  3. Reach the starting room of Chest or Dark Room, pass through the Golden Gate, and defeat Mega Satan. source
  - Tainted Lost
- Tainted Lost → Boss Rush (hard)
  - Tainted Lost
- Tainted Lost → Hush (hard)
  1. Defeat Mom's Heart for the 10th time to unlock Blue Womb. source
  2. Defeat Mom's Heart or It Lives! within 30 minutes and enter the door to Blue Womb. source
  3. Defeat Hush in Blue Womb. source
  - Tainted Lost
- Tainted Lost → Ultra Greedier (hard)
  - Tainted Lost
- Tainted Lost → Delirium (hard)
  1. Defeat Hush to unlock The Void. source
  2. A Void portal can appear after a major boss; defeating Hush guarantees one. source
  3. Defeat Delirium, the main boss of The Void. source
  - Tainted Lost
- Tainted Lost → Mother (hard)
  1. Defeat Hush 3 times to unlock A Secret Exit. source
  2. After A Secret Exit is unlocked, a locked door to Downpour appears after completing Basement/Cellar/Burning Basement I or II. source
  3. Collect Knife Piece 1 in Downpour/Dross II. source
  4. Collect Knife Piece 2 in Mines/Ashpit II. source
  5. After defeating Mom in Mausoleum/Gehenna II, stab the flesh door that appears with the knife familiar to open it. source
  6. Defeat the Mausoleum version of Mom's Heart behind the flesh door, then reach Corpse II and defeat Mother. source
  - Tainted Lost
- Tainted Lost → The Beast (hard)
  1. A Strange Door is unlocked by beating Mother for the first time. source
  2. Take The Polaroid or The Negative after Mom, escape the boss room, then use it to open A Strange Door. source
  3. Find Dad's Note, complete the Ascent to Home, then defeat Dogma, the Ultra Harbingers, and The Beast. source
  - Tainted Lost
- Tainted Lilith → Mom's Heart or It Lives! (hard)
  - Tainted Lilith
- Tainted Lilith → Isaac (hard)
  - Tainted Lilith
- Tainted Lilith → ??? (hard)
  - Tainted Lilith
- Tainted Lilith → Satan (hard)
  - Tainted Lilith
- Tainted Lilith → The Lamb (hard)
  1. Defeat Satan 5 times to unlock The Negative. source
  2. The Negative is a guaranteed drop from Mom; take it, then defeat Satan in Sheol and touch the chest to enter Dark Room. source
  3. Find and defeat The Lamb in Dark Room. source
  - Tainted Lilith
- Tainted Lilith → Mega Satan (hard)
  1. ??? or The Lamb must be defeated once for the Golden Gate to spawn. source
  2. Open the Golden Gate by combining Key Piece 1 and Key Piece 2 into the Golden Key, or by using Dad's Key or another gate-opening effect. source
  3. Reach the starting room of Chest or Dark Room, pass through the Golden Gate, and defeat Mega Satan. source
  - Tainted Lilith
- Tainted Lilith → Boss Rush (hard)
  - Tainted Lilith
- Tainted Lilith → Hush (hard)
  1. Defeat Mom's Heart for the 10th time to unlock Blue Womb. source
  2. Defeat Mom's Heart or It Lives! within 30 minutes and enter the door to Blue Womb. source
  3. Defeat Hush in Blue Womb. source
  - Tainted Lilith
- Tainted Lilith → Ultra Greedier (hard)
  - Tainted Lilith
- Tainted Lilith → Delirium (hard)
  1. Defeat Hush to unlock The Void. source
  2. A Void portal can appear after a major boss; defeating Hush guarantees one. source
  3. Defeat Delirium, the main boss of The Void. source
  - Tainted Lilith
- Tainted Lilith → Mother (hard)
  1. Defeat Hush 3 times to unlock A Secret Exit. source
  2. After A Secret Exit is unlocked, a locked door to Downpour appears after completing Basement/Cellar/Burning Basement I or II. source
  3. Collect Knife Piece 1 in Downpour/Dross II. source
  4. Collect Knife Piece 2 in Mines/Ashpit II. source
  5. After defeating Mom in Mausoleum/Gehenna II, stab the flesh door that appears with the knife familiar to open it. source
  6. Defeat the Mausoleum version of Mom's Heart behind the flesh door, then reach Corpse II and defeat Mother. source
  - Tainted Lilith
- Tainted Lilith → The Beast (hard)
  1. A Strange Door is unlocked by beating Mother for the first time. source
  2. Take The Polaroid or The Negative after Mom, escape the boss room, then use it to open A Strange Door. source
  3. Find Dad's Note, complete the Ascent to Home, then defeat Dogma, the Ultra Harbingers, and The Beast. source
  - Tainted Lilith
- Tainted Keeper → Mom's Heart or It Lives! (hard)
  - Tainted Keeper
- Tainted Keeper → Isaac (hard)
  - Tainted Keeper
- Tainted Keeper → ??? (hard)
  - Tainted Keeper
- Tainted Keeper → Satan (hard)
  - Tainted Keeper
- Tainted Keeper → The Lamb (hard)
  1. Defeat Satan 5 times to unlock The Negative. source
  2. The Negative is a guaranteed drop from Mom; take it, then defeat Satan in Sheol and touch the chest to enter Dark Room. source
  3. Find and defeat The Lamb in Dark Room. source
  - Tainted Keeper
- Tainted Keeper → Mega Satan (hard)
  1. ??? or The Lamb must be defeated once for the Golden Gate to spawn. source
  2. Open the Golden Gate by combining Key Piece 1 and Key Piece 2 into the Golden Key, or by using Dad's Key or another gate-opening effect. source
  3. Reach the starting room of Chest or Dark Room, pass through the Golden Gate, and defeat Mega Satan. source
  - Tainted Keeper
- Tainted Keeper → Boss Rush (hard)
  - Tainted Keeper
- Tainted Keeper → Hush (hard)
  1. Defeat Mom's Heart for the 10th time to unlock Blue Womb. source
  2. Defeat Mom's Heart or It Lives! within 30 minutes and enter the door to Blue Womb. source
  3. Defeat Hush in Blue Womb. source
  - Tainted Keeper
- Tainted Keeper → Ultra Greedier (hard)
  - Tainted Keeper
- Tainted Keeper → Delirium (hard)
  1. Defeat Hush to unlock The Void. source
  2. A Void portal can appear after a major boss; defeating Hush guarantees one. source
  3. Defeat Delirium, the main boss of The Void. source
  - Tainted Keeper
- Tainted Keeper → Mother (hard)
  1. Defeat Hush 3 times to unlock A Secret Exit. source
  2. After A Secret Exit is unlocked, a locked door to Downpour appears after completing Basement/Cellar/Burning Basement I or II. source
  3. Collect Knife Piece 1 in Downpour/Dross II. source
  4. Collect Knife Piece 2 in Mines/Ashpit II. source
  5. After defeating Mom in Mausoleum/Gehenna II, stab the flesh door that appears with the knife familiar to open it. source
  6. Defeat the Mausoleum version of Mom's Heart behind the flesh door, then reach Corpse II and defeat Mother. source
  - Tainted Keeper
- Tainted Keeper → The Beast (hard)
  1. A Strange Door is unlocked by beating Mother for the first time. source
  2. Take The Polaroid or The Negative after Mom, escape the boss room, then use it to open A Strange Door. source
  3. Find Dad's Note, complete the Ascent to Home, then defeat Dogma, the Ultra Harbingers, and The Beast. source
  - Tainted Keeper
- Tainted Apollyon → Mom's Heart or It Lives! (hard)
  - Tainted Apollyon
- Tainted Apollyon → Isaac (hard)
  - Tainted Apollyon
- Tainted Apollyon → ??? (hard)
  - Tainted Apollyon
- Tainted Apollyon → Satan (hard)
  - Tainted Apollyon
- Tainted Apollyon → The Lamb (hard)
  1. Defeat Satan 5 times to unlock The Negative. source
  2. The Negative is a guaranteed drop from Mom; take it, then defeat Satan in Sheol and touch the chest to enter Dark Room. source
  3. Find and defeat The Lamb in Dark Room. source
  - Tainted Apollyon
- Tainted Apollyon → Mega Satan (hard)
  1. ??? or The Lamb must be defeated once for the Golden Gate to spawn. source
  2. Open the Golden Gate by combining Key Piece 1 and Key Piece 2 into the Golden Key, or by using Dad's Key or another gate-opening effect. source
  3. Reach the starting room of Chest or Dark Room, pass through the Golden Gate, and defeat Mega Satan. source
  - Tainted Apollyon
- Tainted Apollyon → Boss Rush (hard)
  - Tainted Apollyon
- Tainted Apollyon → Hush (hard)
  1. Defeat Mom's Heart for the 10th time to unlock Blue Womb. source
  2. Defeat Mom's Heart or It Lives! within 30 minutes and enter the door to Blue Womb. source
  3. Defeat Hush in Blue Womb. source
  - Tainted Apollyon
- Tainted Apollyon → Ultra Greedier (hard)
  - Tainted Apollyon
- Tainted Apollyon → Delirium (hard)
  1. Defeat Hush to unlock The Void. source
  2. A Void portal can appear after a major boss; defeating Hush guarantees one. source
  3. Defeat Delirium, the main boss of The Void. source
  - Tainted Apollyon
- Tainted Apollyon → Mother (hard)
  1. Defeat Hush 3 times to unlock A Secret Exit. source
  2. After A Secret Exit is unlocked, a locked door to Downpour appears after completing Basement/Cellar/Burning Basement I or II. source
  3. Collect Knife Piece 1 in Downpour/Dross II. source
  4. Collect Knife Piece 2 in Mines/Ashpit II. source
  5. After defeating Mom in Mausoleum/Gehenna II, stab the flesh door that appears with the knife familiar to open it. source
  6. Defeat the Mausoleum version of Mom's Heart behind the flesh door, then reach Corpse II and defeat Mother. source
  - Tainted Apollyon
- Tainted Apollyon → The Beast (hard)
  1. A Strange Door is unlocked by beating Mother for the first time. source
  2. Take The Polaroid or The Negative after Mom, escape the boss room, then use it to open A Strange Door. source
  3. Find Dad's Note, complete the Ascent to Home, then defeat Dogma, the Ultra Harbingers, and The Beast. source
  - Tainted Apollyon
- Tainted Forgotten → Mom's Heart or It Lives! (hard)
  - Tainted Forgotten
- Tainted Forgotten → Isaac (hard)
  - Tainted Forgotten
- Tainted Forgotten → ??? (hard)
  - Tainted Forgotten
- Tainted Forgotten → Satan (hard)
  - Tainted Forgotten
- Tainted Forgotten → The Lamb (hard)
  1. Defeat Satan 5 times to unlock The Negative. source
  2. The Negative is a guaranteed drop from Mom; take it, then defeat Satan in Sheol and touch the chest to enter Dark Room. source
  3. Find and defeat The Lamb in Dark Room. source
  - Tainted Forgotten
- Tainted Forgotten → Mega Satan (hard)
  1. ??? or The Lamb must be defeated once for the Golden Gate to spawn. source
  2. Open the Golden Gate by combining Key Piece 1 and Key Piece 2 into the Golden Key, or by using Dad's Key or another gate-opening effect. source
  3. Reach the starting room of Chest or Dark Room, pass through the Golden Gate, and defeat Mega Satan. source
  - Tainted Forgotten
- Tainted Forgotten → Boss Rush (hard)
  - Tainted Forgotten
- Tainted Forgotten → Hush (hard)
  1. Defeat Mom's Heart for the 10th time to unlock Blue Womb. source
  2. Defeat Mom's Heart or It Lives! within 30 minutes and enter the door to Blue Womb. source
  3. Defeat Hush in Blue Womb. source
  - Tainted Forgotten
- Tainted Forgotten → Ultra Greedier (hard)
  - Tainted Forgotten
- Tainted Forgotten → Delirium (hard)
  1. Defeat Hush to unlock The Void. source
  2. A Void portal can appear after a major boss; defeating Hush guarantees one. source
  3. Defeat Delirium, the main boss of The Void. source
  - Tainted Forgotten
- Tainted Forgotten → Mother (hard)
  1. Defeat Hush 3 times to unlock A Secret Exit. source
  2. After A Secret Exit is unlocked, a locked door to Downpour appears after completing Basement/Cellar/Burning Basement I or II. source
  3. Collect Knife Piece 1 in Downpour/Dross II. source
  4. Collect Knife Piece 2 in Mines/Ashpit II. source
  5. After defeating Mom in Mausoleum/Gehenna II, stab the flesh door that appears with the knife familiar to open it. source
  6. Defeat the Mausoleum version of Mom's Heart behind the flesh door, then reach Corpse II and defeat Mother. source
  - Tainted Forgotten
- Tainted Forgotten → The Beast (hard)
  1. A Strange Door is unlocked by beating Mother for the first time. source
  2. Take The Polaroid or The Negative after Mom, escape the boss room, then use it to open A Strange Door. source
  3. Find Dad's Note, complete the Ascent to Home, then defeat Dogma, the Ultra Harbingers, and The Beast. source
  - Tainted Forgotten
- Tainted Bethany → Mom's Heart or It Lives! (hard)
  - Tainted Bethany
- Tainted Bethany → Isaac (hard)
  - Tainted Bethany
- Tainted Bethany → ??? (hard)
  - Tainted Bethany
- Tainted Bethany → Satan (hard)
  - Tainted Bethany
- Tainted Bethany → The Lamb (hard)
  1. Defeat Satan 5 times to unlock The Negative. source
  2. The Negative is a guaranteed drop from Mom; take it, then defeat Satan in Sheol and touch the chest to enter Dark Room. source
  3. Find and defeat The Lamb in Dark Room. source
  - Tainted Bethany
- Tainted Bethany → Mega Satan (hard)
  1. ??? or The Lamb must be defeated once for the Golden Gate to spawn. source
  2. Open the Golden Gate by combining Key Piece 1 and Key Piece 2 into the Golden Key, or by using Dad's Key or another gate-opening effect. source
  3. Reach the starting room of Chest or Dark Room, pass through the Golden Gate, and defeat Mega Satan. source
  - Tainted Bethany
- Tainted Bethany → Boss Rush (hard)
  - Tainted Bethany
- Tainted Bethany → Hush (hard)
  1. Defeat Mom's Heart for the 10th time to unlock Blue Womb. source
  2. Defeat Mom's Heart or It Lives! within 30 minutes and enter the door to Blue Womb. source
  3. Defeat Hush in Blue Womb. source
  - Tainted Bethany
- Tainted Bethany → Ultra Greedier (hard)
  - Tainted Bethany
- Tainted Bethany → Delirium (hard)
  1. Defeat Hush to unlock The Void. source
  2. A Void portal can appear after a major boss; defeating Hush guarantees one. source
  3. Defeat Delirium, the main boss of The Void. source
  - Tainted Bethany
- Tainted Bethany → Mother (hard)
  1. Defeat Hush 3 times to unlock A Secret Exit. source
  2. After A Secret Exit is unlocked, a locked door to Downpour appears after completing Basement/Cellar/Burning Basement I or II. source
  3. Collect Knife Piece 1 in Downpour/Dross II. source
  4. Collect Knife Piece 2 in Mines/Ashpit II. source
  5. After defeating Mom in Mausoleum/Gehenna II, stab the flesh door that appears with the knife familiar to open it. source
  6. Defeat the Mausoleum version of Mom's Heart behind the flesh door, then reach Corpse II and defeat Mother. source
  - Tainted Bethany
- Tainted Bethany → The Beast (hard)
  1. A Strange Door is unlocked by beating Mother for the first time. source
  2. Take The Polaroid or The Negative after Mom, escape the boss room, then use it to open A Strange Door. source
  3. Find Dad's Note, complete the Ascent to Home, then defeat Dogma, the Ultra Harbingers, and The Beast. source
  - Tainted Bethany
- Tainted Jacob → Mom's Heart or It Lives! (hard)
  - Tainted Jacob
- Tainted Jacob → Isaac (hard)
  - Tainted Jacob
- Tainted Jacob → ??? (hard)
  - Tainted Jacob
- Tainted Jacob → Satan (hard)
  - Tainted Jacob
- Tainted Jacob → The Lamb (hard)
  1. Defeat Satan 5 times to unlock The Negative. source
  2. The Negative is a guaranteed drop from Mom; take it, then defeat Satan in Sheol and touch the chest to enter Dark Room. source
  3. Find and defeat The Lamb in Dark Room. source
  - Tainted Jacob
- Tainted Jacob → Mega Satan (hard)
  1. ??? or The Lamb must be defeated once for the Golden Gate to spawn. source
  2. Open the Golden Gate by combining Key Piece 1 and Key Piece 2 into the Golden Key, or by using Dad's Key or another gate-opening effect. source
  3. Reach the starting room of Chest or Dark Room, pass through the Golden Gate, and defeat Mega Satan. source
  - Tainted Jacob
- Tainted Jacob → Boss Rush (hard)
  - Tainted Jacob
- Tainted Jacob → Hush (hard)
  1. Defeat Mom's Heart for the 10th time to unlock Blue Womb. source
  2. Defeat Mom's Heart or It Lives! within 30 minutes and enter the door to Blue Womb. source
  3. Defeat Hush in Blue Womb. source
  - Tainted Jacob
- Tainted Jacob → Ultra Greedier (hard)
  - Tainted Jacob
- Tainted Jacob → Delirium (hard)
  1. Defeat Hush to unlock The Void. source
  2. A Void portal can appear after a major boss; defeating Hush guarantees one. source
  3. Defeat Delirium, the main boss of The Void. source
  - Tainted Jacob
- Tainted Jacob → Mother (hard)
  1. Defeat Hush 3 times to unlock A Secret Exit. source
  2. After A Secret Exit is unlocked, a locked door to Downpour appears after completing Basement/Cellar/Burning Basement I or II. source
  3. Collect Knife Piece 1 in Downpour/Dross II. source
  4. Collect Knife Piece 2 in Mines/Ashpit II. source
  5. After defeating Mom in Mausoleum/Gehenna II, stab the flesh door that appears with the knife familiar to open it. source
  6. Defeat the Mausoleum version of Mom's Heart behind the flesh door, then reach Corpse II and defeat Mother. source
  - Tainted Jacob
- Tainted Jacob → The Beast (hard)
  1. A Strange Door is unlocked by beating Mother for the first time. source
  2. Take The Polaroid or The Negative after Mom, escape the boss room, then use it to open A Strange Door. source
  3. Find Dad's Note, complete the Ascent to Home, then defeat Dogma, the Ultra Harbingers, and The Beast. source
  - Tainted Jacob

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What unlocking Death Certificate leads to

Source: bindingofisaacrebirth.wiki.gg

Imported 2026-09-13